Soluble Protein Assay to Quantify Soluble Protein Content in Plant Tissues
Published: April 30, 2023
Abstract
Source: Deans, C. A. et al. Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar. J. Vis. Exp. (2018)
This video describes a colorimetric method to quantify the soluble protein content in plant tissues. The method uses Coomassie Brilliant Blue G-250 dye, which interacts with the soluble protein to form a blue-colored stable protein-dye complex, proportional to the soluble protein content in the tissue.
Protocol
1. Plant Collection and Processing Collect and process plant samples After collecting plant samples, flash-freeze samples by dipping plant material into liquid nitrogen with forceps and store at -80 °C. If the plant samples collected are too large to flash-freeze, quickly cool the samples using dry ice and transfer to a -80 °C freezer as soon as possible. The macronutrient content of plant material can change after tissues are separated from the plant, so it is important to freeze plant …
Declarações
The authors have nothing to disclose.
Materials
| Microplate reader (spectrophotometer) | Bio-Rad | Model 680 XR | |
| Bio-Rad Protein Assay Dye Reagent concentrate | Bio-Rad | #5000006 | 450mL |
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Soluble Protein Assay to Quantify Soluble Protein Content in Plant Tissues. J. Vis. Exp. (Pending Publication), e21273, doi: (2023).