Endotoxin Activity Assay: A Chemiluminescence-Based Bioassay for Investigating Endotoxin Activity in Blood Sample
Published: April 30, 2023
Abstract
Source: Pinciroli, R. et al. Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients. J. Vis. Exp. (2019).
This video demonstrates a rapid bioassay for investigating endotoxin activity in a whole blood sample via a chemiluminescence-based method. This assay is a rapid and sensitive biomarker test for early-stage diagnosis of endotoxin-mediated sepsis.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Endotoxin Activity Assay (EAA) Collect patient blood samples in sterile tubes containing EDTA anticoagulant. Store blood samples at room temperature Prepare the EA test tubes for each patient's blood sample you need to test. Put …
Declarações
The authors have nothing to disclose.
Materials
| EAA kit | Spectral Medical Inc. | EAAST-20 | Package with 20 tests + 1 quality control |
| Smart Line TL | Berthold | EAASL | Luminometer |
| Incubator shaker | GRANT | ES-20 | Mini-incubator shaker |
| Vortexer | VWR | 444-2790 | Vortex instrument |
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Endotoxin Activity Assay: A Chemiluminescence-Based Bioassay for Investigating Endotoxin Activity in Blood Sample. J. Vis. Exp. (Pending Publication), e21173, doi: (2023).