Prepping Conscious Rabbit Model for Simultaneous Video-EEG-ECG-Oximetry-Capnography for Experimental Interventions: A Procedure to Implant EEE-ECG Electrodes and Attach Respiratory Probes in Rabbit Model
Published: April 30, 2023
Abstract
Source: Bosinski, C. et al., Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits. J. Vis. Exp. (2021).
This video demonstrates the procedure to implant EEG-ECG electrodes and attach respiratory probes in a rabbit model for acquiring and analyzing records of its bio signals.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Implanting EEG-ECG electrodes and attaching respiratory monitors Remove one rabbit from the transport carrier and place in the lap of a seated investigator. Hold the rabbit vertically and keep it close to the investigator's body. Lower the rabbit into a supine position, with the rabbit's…
Representative Results
Figure 1: Picture of EEG and ECG electrodes. (A) Bent ECG electrodes and straight EEG electrodes. (B) How to hook the ECG electrode in the subcutaneous tissue of the rabbit, so that it is through and through. Abbreviations (LL: Left limb, RA: Right arm, RL: Right limb, LA: Left arm, RF: Right frontal, LF: Left frontal, Cz: Center, RO: Right occipital, LO: Left Oc…
Declarações
The authors have nothing to disclose.
Materials
| Computer | Dell | Optiplex 5040 | Acquisition computer |
| ECG Electrode | RhythmLink | RLSND116-2.5 | 13mm 35-degree bent (0.4 mm diameter) subdermal pin electrodes |
| EEG Electrode | RhythmLink | RLSP513 | 5-twist 13mm straight (0.4mm diameter) subdermal pin electrodes |
| EEGLAB (2020) | Swartz Center for Computational Neuroscience | Open Access | Can perform spectral analysis of EEG |
| Ethernet-to-ethernet adapter | Linksys | USB3G16 | Adapter for connecting the camera to the computer |
| Foam padding | Generic | N/A | Reduces pressure applied to the neck of small rabbits by the restrainer in order to prevent the adverse cardiorespiratory effects of neck compression |
| MATLAB (R2019b, Update 5) | MathWorks | N/A | Required to run EEGLAB |
| Microphone | Sony Stereo | ECM-D570P | Recording of audible manifestions of seizures |
| Micropore Medical Tape, Paper, White | 3M | 1530-1 | Used to secure wires and create ear splint |
| Natus NeuroWorks | Natus | LC101-8 | Acquisition and review software |
| Photic Stimulator | Grass | PS22 | Stimulator to control frequency, delay, duration, intensity of the light pulses |
| Plastic wire organizer / bundler | 12Vwire.com | LM-12-100-BLK | Bundle wires to cut down on noise |
| PS 22 Photic Stimulator | Grass Instruments | BZA641035 | Strobe light with adjustable flash frequency, delay, and intensity |
| PVC pipe | Generic | N/A | Prevents small rabbits from kicking their hind legs and causing spinal injury |
| Rabbit Restrainer | Plas-Labs | 501-TC | Various size rabbit restrainers are available. 6" x 18" x 6" in this study. |
| Rubber pad (booster) | Generic | N/A | Raises small rabbits up in the restrainer to prevent neck compression |
| SpO2 ear clip | NONIN | 61000 | PureSAT/SpO2 |
| SpO2 sensor adapter | NONIN | 13931 | XPOD PureSAT/SpO2 |
| Webcol Alcohol Prep, Sterile, Large, 2-ply | Covidien | 5110 | To prepare ear vein before catheterization |
| LabChart Pro (2019, Version 8.1.16) | ADInstruments | N/A | ECG Analysis |
| Quantum Amplifier | Natus | 13926 | Amplifier / digitizer |
| Quantum HeadBox Amplifier | Natus | 22134 | 64-pin breakout box |
| SRG-X120 1080p PTZ Camera with HDMI, IP & 3G-SDI Output | Sony | SRG-X120 | Impela Camera |
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Prepping Conscious Rabbit Model for Simultaneous Video-EEG-ECG-Oximetry-Capnography for Experimental Interventions: A Procedure to Implant EEE-ECG Electrodes and Attach Respiratory Probes in Rabbit Model. J. Vis. Exp. (Pending Publication), e20823, doi: (2023).