Surgical Induction of Lumbar Spine Instability in Mouse Model: A Surgical Procedure for the Resection of the Lumbar Spinous Processes and Connected Ligaments in Murine Model

Published: April 30, 2023

Abstract

Source: Liu, S. et al. A Mouse Model of Lumbar Spine Instability. J. Vis. Exp. (2021)

In this video, we describe the surgical procedure to induce lumbar spine instability in a mouse model by resecting the Lumbar L3 to L5 spinous process along with supraspinous and interspinous ligaments.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Pre-operation preparation Instrument sterilization: Steam-sterilize surgical instruments in an autoclave (121 °C for 15 min) prior to the surgery. Pack instruments in a metal container and maintain them until they are used in the surgery. Surgery platform setup: Assign a bench area of at least 60 cm x 60 …

Representative Results

Figure 1: Exposure of L3–L5 spinous processes and LSI operation. (A) A custom-made cylindrical pad is placed under the mouse’s abdomen. (B) Exposure of the lumbar fasciae and identification of L3 to S1 spinous processes by “V” shapes. (C) Lateral paraspinous muscle incisions on both sides of L3 to L5 spino…

Declarações

The authors have nothing to disclose.

Materials

Chlortetracycline Hydrochloride Eye Ointment Shanghai General Pharmaceutical Co., Ltd. H31021931 Prevent eye dry, Prevent wound infection
C57BL/6J male mice Tian-jiang Pharmaceuticals Company (Jiangsu, CN) SCXK2018-0004 Animal model
Disposable medical towel Henan Huayu Medical Devices Co., Ltd. 20160090 Platform for surgical operation
Inhalant anesthesia equipment MIDMARK Matrx 3000 Anesthesia
Isoflurane Shenzhen RWD Life Technology Co., Ltd. 1903715 Anesthesia
Lidocaine hydrochloride Shandong Hualu Pharmaceutical Co., Ltd. H37022839 Pain relief
Medical suture needle Shanghai Pudong Jinhuan Medical Products Co., Ltd. 20S0401J Suture skin
Ophthalmic forceps Shanghai Medical Devices (Group) Co., Ltd. Surgical Instruments Factory JD1050 Clip the skin
Ophthalmic scissors(10cm) Shanghai Medical Devices (Group) Co., Ltd. Surgical Instruments Factory Y00030 Skin incision
Silk braided Shanghai Pudong Jinhuan Medical Products Co., Ltd. 11V0820 Suture skin
Small animal trimmer Shanghai Feike Electric Co., Ltd. FC5910 Hair removal
Sterile surgical blades(12#) Shanghai Pudong Jinhuan Medical Products Co., Ltd. 35T0707 Muscle incision
Veet hair removal cream RECKITT BENCKISER (India) Ltd NA Hair removal
Venus shears Mingren medical equipment Length:12.5cm Clip the muscle and spinous process

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Surgical Induction of Lumbar Spine Instability in Mouse Model: A Surgical Procedure for the Resection of the Lumbar Spinous Processes and Connected Ligaments in Murine Model. J. Vis. Exp. (Pending Publication), e20808, doi: (2023).

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