This video describes the dissection of the accessory glands from male flies and shows an example in which the isolated gland will be dissociated into a single cell suspension.
Protocol
This protocol is an excerpt from Immarigeon et al., A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands, J. Vis. Exp. (2019). 1. Solutions and Material Preparation Prepare the following solutions. Prepare Serum Supplemented Medium (SSM): Schneider's Drosophila medium complemented with 10% heat inactivated fetal bovine serum and 1% Penicillin-Streptomycin.</li…