JOVE JOURNAL

Genética

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Journal (Genetics)

DNA Extraction from Cancer Tissues for the Detection of Low-Frequency Mutations Using Blocker Displacement Amplification Technology

Haixia Xu*¹, Junyan Lu*², Zhou Li¹, Ran Chen¹

¹Kindstar Global Technology Inc., ²Yongzhou Central Hospital

Journal (Genetics)

PCR Amplification for Sensitive Detection of Tumor Mutations in the Extracted Cancer DNA Samples

Haixia Xu*¹, Junyan Lu*², Zhou Li¹, Ran Chen¹

¹Kindstar Global Technology Inc., ²Yongzhou Central Hospital

Journal (Genetics)

Blocker Displacement Amplification-Enhanced Sanger Sequencing for Low-Frequency Mutation Detection in Cancer Tissues

Haixia Xu*¹, Junyan Lu*², Zhou Li¹, Ran Chen¹

¹Kindstar Global Technology Inc., ²Yongzhou Central Hospital

Journal (Genetics)

Quantification Pipeline for Evaluating Protein Distribution with Varied Nuclear Organization Patterns in Human T Lymphocytes

Valeria Di Gioia^1,2, Jan Zamporlini^1,3, Rebecca Vadalà^1,3, Elena Parmigiani⁴, Beatrice Bodega^1,3, Federica Marasca¹

¹Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi" (INGM), ²SEMM, European School of Molecular Medicine, ³Department of Biosciences,University of Milan, ⁴T-One Therapeutics Srl

Journal (Genetics)

Embryo Microinjection for Transgenesis in Drosophila

Guang Chen*^1,2, Xinyi Zhang*^1,3, Si Xu^1,4, Xiaotao Zhou¹, Wen Xue¹, Xuelian Liu¹, Jialong Yan¹, Nana Zhang¹, Jiwu Wang^1,2,4

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Institute of Biochemistry and Molecular Biology, Hengyang Medical School,University of South China, ⁴Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China

Journal (Genetics)

Preparation and Collection of Drosophila Embryos for Transgenesis

Guang Chen*^1,2, Xinyi Zhang*^1,3, Si Xu^1,4, Xiaotao Zhou¹, Wen Xue¹, Xuelian Liu¹, Jialong Yan¹, Nana Zhang¹, Jiwu Wang^1,2,4

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Institute of Biochemistry and Molecular Biology, Hengyang Medical School,University of South China, ⁴Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China

Journal (Genetics)

Microinjection of Drosophila Embryos for phiC31 Integrase Mediated Embryo Transgenesis

Guang Chen*^1,2, Xinyi Zhang*^1,3, Si Xu^1,4, Xiaotao Zhou¹, Wen Xue¹, Xuelian Liu¹, Jialong Yan¹, Nana Zhang¹, Jiwu Wang^1,2,4

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Institute of Biochemistry and Molecular Biology, Hengyang Medical School,University of South China, ⁴Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China

Journal (Genetics)

Large-Scale Construction of UAS-cDNA/ORF Plasmids by CRISPR-Based Modular Assembly (CRISPRmass)

Si Xu*^1,2, Mengyu Chen*^1,2, Guang Chen^1,3, Si Luo⁴, Wen Xue^1,2, Xuelian Liu^1,2, Jiwu Wang^1,2,3, Ping Wei⁴

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ⁴Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Journal (Genetics)

Large-Scale Transformation of UAS-cDNA/ORF Plasmids from CRISPR-Based Modular Assembly into E. coli

Si Xu*^1,2, Mengyu Chen*^1,2, Guang Chen^1,3, Si Luo⁴, Wen Xue^1,2, Xuelian Liu^1,2, Jiwu Wang^1,2,3, Ping Wei⁴

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ⁴Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Journal (Genetics)

CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library

Si Xu*^1,2, Mengyu Chen*^1,2, Guang Chen^1,3, Si Luo⁴, Wen Xue^1,2, Xuelian Liu^1,2, Jiwu Wang^1,2,3, Ping Wei⁴

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ⁴Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Journal (Genetics)

Monochrome Multiplex Quantitative PCR (MMqPCR) Data Analysis to Check the Reproducibility of Telomere Length Measurements

Noelle A. Martin*¹, Lauren W. Y. McLester-Davis*^2,3,4, Trevor R. Roy¹, Madeline G. Magruder⁵, Waylon J. Hastings¹, Stacy S. Drury⁶

¹School of Medicine,Tulane University, ²Native American Center for Health Professions,University of Wisconsin-Madison, ³Department of Medicine,University of Wisconsin-Madison, ⁴Department of Biochemistry,University of Wisconsin-Madison, ⁵School of Science and Engineering,Tulane University, ⁶Department of Psychiatry and Behavioral Sciences,Boston Children’s Hospital

Journal (Genetics)

Sample and Master Mix Preparation for Telomere Length Measurement Using Monochrome Multiplex Quantitative PCR (MMqPCR)

Noelle A. Martin*¹, Lauren W. Y. McLester-Davis*^2,3,4, Trevor R. Roy¹, Madeline G. Magruder⁵, Waylon J. Hastings¹, Stacy S. Drury⁶

¹School of Medicine,Tulane University, ²Native American Center for Health Professions,University of Wisconsin-Madison, ³Department of Medicine,University of Wisconsin-Madison, ⁴Department of Biochemistry,University of Wisconsin-Madison, ⁵School of Science and Engineering,Tulane University, ⁶Department of Psychiatry and Behavioral Sciences,Boston Children’s Hospital

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