Isolation and Genotyping of Gastrulating Mouse Embryo for Single-Cell Sequencing
1Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center,Temple University, Lewis Katz School of Medicine, 2Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, 3Department of Child and Adolescence Psychiatry,Children’s Hospital of Philadelphia
Summary
This paper establishes a pipeline for high-quality single-cell and nuclei suspensions of gastrulating mouse embryos for sequencing of single cells and nuclei.
