Journal
Biologia
High-Resolution Analysis of Mitochondrial Function in Human PBMCs

High-Resolution Analysis of Mitochondrial Function in Human PBMCs

May 24, 2024

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

01:24 min
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399 Views

Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics

05:58 min
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132 Views
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1Division of Metabolism, Endocrinology and Nutrition, Department of Medicine,University of Washington, 2Department of Radiology,University of Washington, 3Department of Laboratory Medicine and Pathology,University of Washington

Summary

Methods for studying mitochondrial bioenergetics under physiologically relevant substrate concentrations in immune cells are limited. We provide a detailed protocol that uses high-resolution fluorespirometry to assess changes in the response of the mitochondrial membrane potential to energy demand in human T-cells, monocytes, and peripheral mononuclear cells.

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