Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Yi-Kai Liu; Zhuojun Luo; Anton Iliuk; Weiguo Andy Tao

doi:10.3791/65844

Journal

Bioquímica

Enhancing EV Isolation for Precise Biofluid Analysis

October 27, 2023

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

01:49 min

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1,846 Views

Extracellular Vesicle (EV) Isolation from Human Urine Samples Using the EVtrap Approach

01:54 min

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208 Views

Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis

02:56 min

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186 Views

Proteome/Phosphoproteome Analysis of the Extracellular Vesicle (EV) Samples Using LC-MS/MS

02:58 min

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217 Views

Yi-Kai Liu¹, Zhuojun Luo¹, Anton Iliuk^1,2, Weiguo Andy Tao^1,2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

Summary

The present protocol provides detailed descriptions for the efficient isolation of urinary extracellular vesicles utilizing functionalized magnetic beads. Moreover, it encompasses subsequent analyses, including western blotting, proteomics, and phosphoproteomics.