Analysis of Transgenerational Epigenetic Inheritance in <em>C. elegans</em> Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

Chengyin Li; Phoebe A. W. Bhagoutie; Victor Lao; Arneet L. Saltzman

doi:10.3791/65285

Journal

Genética

Epigenetic Inheritance and ChIP in Caenorhabditis elegans

Epigenetic Inheritance and ChIP in Caenorhabditis elegans

May 05, 2023

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

01:27 min

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1,212 Views

Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates

02:36 min

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Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression

02:02 min

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Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP)

00:59 min

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Formaldehyde Crosslinking of the F1 Generation of Caenorhabditis elegans for ChIP

01:18 min

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Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation

02:41 min

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Chengyin Li*¹, Phoebe A. W. Bhagoutie*¹, Victor Lao¹, Arneet L. Saltzman¹

¹Department of Cell and Systems Biology,University of Toronto

Summary

This protocol describes an RNA interference and ChIP assay to study the epigenetic inheritance of RNAi-induced silencing and associated chromatin modifications in C. elegans.