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Epigenetic Inheritance and ChIP in Caenorhabditis elegans

Epigenetic Inheritance and ChIP in Caenorhabditis elegans

May 05, 2023

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

01:27 min
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1,259 Views

Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates

02:36 min
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Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression

02:02 min
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Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP)

00:59 min
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Formaldehyde Crosslinking of the F1 Generation of Caenorhabditis elegans for ChIP

01:18 min
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Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation

02:41 min
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1Department of Cell and Systems Biology,University of Toronto

概要

This protocol describes an RNA interference and ChIP assay to study the epigenetic inheritance of RNAi-induced silencing and associated chromatin modifications in C. elegans.

Article