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์‹ ๊ฒฝ๊ณผํ•™
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์ธ๊ฐ„ ์ค„๊ธฐ ์„ธํฌ ์ด์‹ ๋ฐ ๊ฒ€์ฆ์„ ์œ„ํ•œ ์ƒ์ฒด ์™ธ ๋ชจ๋ธ๋กœ์„œ์˜ ์„ฑ์ธ ์ธ๊ฐ„ ํ”ผ์งˆ์˜ ๊ธฐ๊ด€ํ˜• ๋ฐฐ์–‘
JoVE ์‹ ๋ฌธ
์‹ ๊ฒฝ๊ณผํ•™
JoVE ๋น„๋””์˜ค๋ฅผ ํ™œ์šฉํ•˜์‹œ๋ ค๋ฉด ๋„์„œ๊ด€์„ ํ†ตํ•œ ๊ธฐ๊ด€ ๊ตฌ๋…์ด ํ•„์š”ํ•ฉ๋‹ˆ๋‹ค.  ์ „์ฒด ๋น„๋””์˜ค๋ฅผ ๋ณด์‹œ๋ ค๋ฉด ๋กœ๊ทธ์ธํ•˜๊ฑฐ๋‚˜ ๋ฌด๋ฃŒ ํŠธ๋ผ์ด์–ผ์„ ์‹œ์ž‘ํ•˜์„ธ์š”.
JoVE ์‹ ๋ฌธ ์‹ ๊ฒฝ๊ณผํ•™
Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
Please note that all translations are automatically generated. Click here for the English version.

์ธ๊ฐ„ ์ค„๊ธฐ ์„ธํฌ ์ด์‹ ๋ฐ ๊ฒ€์ฆ์„ ์œ„ํ•œ ์ƒ์ฒด ์™ธ ๋ชจ๋ธ๋กœ์„œ์˜ ์„ฑ์ธ ์ธ๊ฐ„ ํ”ผ์งˆ์˜ ๊ธฐ๊ด€ํ˜• ๋ฐฐ์–‘

DOI:
10.3791/64234-v

โ€ข

07:16 min

โ€ข

December 09, 2022

โ€ข

, , , ,
1Laboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center,Lund University, 2Lund Stem Cell Center,Lund University

Chapters

  • 00:05Introduction
  • 01:14Tissue Collection, Maintenance, Cutting, and Plating
  • 03:25Transplantation of the GFP-lt-NES Cells into Organotypic Human Adult Cortical (hACtx) Slices
  • 04:57Results: Characterization and Electrophysiological Properties of hACtx Neurons
  • 06:49Conclusion

Summary

์ž๋™ ๋ฒˆ์—ญ

์ž๋™ ๋ฒˆ์—ญ

์ด ํ”„๋กœํ† ์ฝœ์€ ์œ ๋„ ๋งŒ๋Šฅ ์ค„๊ธฐ ์„ธํฌ ์œ ๋ž˜ ํ”ผ์งˆ ์ „๊ตฌ์ฒด์˜ ์ƒ์ฒด ์™ธ ํ”ผ์งˆ ๋‚ด ์ด์‹๊ณผ ๊ฒฐํ•ฉ๋œ ์„ฑ์ธ ์ธ๊ฐ„ ํ”ผ์งˆ์˜ ์žฅ๊ธฐ ๊ธฐ๊ด€ํ˜• ๋ฐฐ์–‘์„ ์„ค๋ช…ํ•˜๋ฉฐ, ์ด๋Š” ์ธ๊ฐ„ ์‹ ๊ฒฝํ‡ดํ–‰์„ฑ ์žฅ์• ์— ๋Œ€ํ•œ ์ค„๊ธฐ ์„ธํฌ ๊ธฐ๋ฐ˜ ์น˜๋ฃŒ๋ฒ•์„ ์ถ”๊ฐ€๋กœ ํ…Œ์ŠคํŠธํ•˜๊ธฐ ์œ„ํ•œ ์ƒˆ๋กœ์šด ๋ฐฉ๋ฒ•๋ก ์„ ์ œ์‹œํ•ฉ๋‹ˆ๋‹ค.

Tags

Keywords: Organotypic CulturesAdult Human CortexEx Vivo ModelHuman Stem Cell TransplantationNeural CircuitryBrain DamageVibratomeCutting SolutionCoronal SlicesSagittal SlicesRinsing Solution

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