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Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
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神经科学
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JoVE 杂志 神经科学
Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation

Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation

2,147 Views

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07:16 min

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December 09, 2022

DOI:

10.3791/64234-v

DOI:
10.3791/64234-v

•

07:16 min
December 09, 2022

•

30 Views
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1Laboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center,Lund University, 2Lund Stem Cell Center,Lund University

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This protocol describes long-term organotypic cultures of adult human cortex combined with ex vivo intracortical transplantation of induced pluripotent stem cell-derived cortical progenitors, which present a novel methodology to further test stem cell-based therapies for human neurodegenerative disorders.

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Palma-Tortosa, S., Martínez-Curiel, R., Aretio-Medina, C., Avaliani, N., Kokaia, Z. Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation. J. Vis. Exp. (190), e64234, doi:10.3791/64234 (2022).

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