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立体定位手术方法 ，通过 大水池在小鼠中显微注射尾部脑干和上颈椎脊髓

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Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice

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立体定位手术方法，通过大水池在小鼠中显微注射尾部脑干和上颈椎脊髓

DOI:

10.3791/63344-v

•

07:07 min

•

January 21, 2022

•

Krutika Joshi, Alana Kirby, Jianguo Niu, Veronique VanderHorst

¹Beth Israel Deaconess Medical Center,Harvard Medical School

Chapters

00:04Introduction
00:52Positioning of the Mouse in the Stereotaxic Frame
02:04Surgery to Access the Cisterna Magna
03:34Opening of the Cisternal Membrane and Identification of Landmarks and Zero Point
04:11Injection of the Target
04:45Closure of the Surgical Field
05:10Results: Stereotaxic Surgical Approach in Mice
06:14Conclusion

Summary

자동 번역

针对小鼠大脑部位的立体定向手术通常涉及通过颅骨进入，并由颅骨特征引导。在这里，我们概述了一种替代立体定位方法，通过大水箱靶向尾部脑干和上颈椎脊髓，该方法依赖于脑干特征的直接可视化。

Tags

Keywords: Stereotaxic Microinjection Caudal Brainstem Upper Cervical Spinal Cord Cisterna Magna Mice Neurosurgical Technique Precision Delivery Restricted Regions Of Interest Reproducible Access Neuroscience Research

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