Journal
/
Bioengineering
/
2D and 3D Matrices to Study Linear Invadosome Formation and Activity
JoVE Journal
Bioengineering
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
2D and 3D Matrices to Study Linear Invadosome Formation and Activity

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

DOI:
10.3791/54911-v

•

12:25 min

•

June 02, 2017

•

, , , , , ,
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai,Tisch Cancer Institute, 3Faculté de Médecine, Casablanca,Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence

Chapters

  • 00:00Title
  • 02:13Preparation of a Collagen-gelatin Mixed Matrix
  • 05:06Immunofluorescence and Linear Invadosome Quantification
  • 07:263D Collagen Invasion Assay
  • 11:19Results
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to prepare a 2D mixed matrix, consisting of gelatin and collagen I, and a 3D collagen I plug to study linear invadosomes. These protocols allow for the study of linear invadosome formation, matrix degradation activity, and the invasion capabilities of primary cells and cancer cell lines.

Tags

Keywords: 2D Matrix3D MatrixLinear InvadosomeExtracellular MatrixCell-matrix InteractionInvadosomesPodosomesInvadopodiaMetalloproteinasesMMP2MT1-MMPType 1 Collagen FibrilsZymographyGelatinMixed Matrix3D Collagen Invasion Assay

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/kr/54911?article_type=v

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
check_url/kr/54911?article_type=v

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
check_url/kr/54911?article_type=v

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
check_url/kr/54911?article_type=v

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
check_url/kr/54911?article_type=v

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
check_url/kr/54911?article_type=v

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
check_url/kr/54911?article_type=v

Novel Process for 3D Printing Decellularized Matrices
check_url/kr/54911?article_type=v

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
check_url/kr/54911?article_type=v

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
check_url/kr/54911?article_type=v

Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels (Video) | JoVE

Read Article