JoVE ๋น๋์ค๋ฅผ ํ์ฉํ์๋ ค๋ฉด ๋์๊ด์ ํตํ ๊ธฐ๊ด ๊ตฌ๋
์ด ํ์ํฉ๋๋ค. ์ ์ฒด ๋น๋์ค๋ฅผ ๋ณด์๋ ค๋ฉด ๋ก๊ทธ์ธํ๊ฑฐ๋ ๋ฌด๋ฃ ํธ๋ผ์ด์ผ์ ์์ํ์ธ์.
Chapters
- 00:05Title
- 01:29Preparing and Fixing Tissues
- 03:04Freezing and Cryosectioning
- 04:26Tyramide Signal Amplification (TSA) for Tracer Visualization
- 08:14Development of the Neural Circuits Controlling the Reproductive Axis
- 10:06Conclusion
Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.
