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Voorwaardelijke Genetische transsynaptische Tracing in de embryonale hersenen van muizen
JoVE 신문
신경과학
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JoVE 신문 신경과학
Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
DOI:

11:03 min

December 22, 2014

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Chapters

  • 00:05Title
  • 01:29Preparing and Fixing Tissues
  • 03:04Freezing and Cryosectioning
  • 04:26Tyramide Signal Amplification (TSA) for Tracer Visualization
  • 08:14Development of the Neural Circuits Controlling the Reproductive Axis
  • 10:06Conclusion

Summary

자동 번역

Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.

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