Journal
/
화학
/
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

DOI:
10.3791/51441-v

•

10:56 min

•

May 30, 2014

•

, , ,
1Department of Chemical and Materials Engineering,University of Alberta, 2Department of Chemical and Petroleum Engineering,University of Calgary

Chapters

  • 00:05Title
  • 01:52Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings
  • 02:52Gravimetric Quantification of Neutral Lipids by Hexane Extraction
  • 04:48Fluorometric Quantification of Neutral Lipids Using Nile Red
  • 06:42Fluorescence Microscopy Technique
  • 08:04Results: Images and Calibration Curves of A. protothecoides Stained with Nile Red
  • 10:18Conclusion

Summary

자동 번역

자동 번역

A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

Tags

Keywords: Algal CellsNeutral LipidsFluorescenceNile RedAuxenochlorella ProtothecoidesLipid ContentBioprocess MonitoringRapid ProtocolGravimetric MethodsCell Membrane Permeabilization

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/51441?article_type=v

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
check_url/kr/51441?article_type=v

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
check_url/kr/51441?article_type=v

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
check_url/kr/51441?article_type=v

A Simple and Efficient Protocol for the Catalytic Insertion Polymerization of Functional Norbornenes
check_url/kr/51441?article_type=v

A Protocol for Safe Lithiation Reactions Using Organolithium Reagents
check_url/kr/51441?article_type=v

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
check_url/kr/51441?article_type=v

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
check_url/kr/51441?article_type=v

Quantifying X-Ray Fluorescence Data Using MAPS
check_url/kr/51441?article_type=v

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction
check_url/kr/51441?article_type=v

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Read Article