Journal
/
생화학
/
Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles
/
精製ヘテロ二量体ABCG5/G8タンパク質の還元的メチル化と結晶化前処理
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein
Please note that all translations are automatically generated. Click here for the English version.

精製ヘテロ二量体ABCG5/G8タンパク質の還元的メチル化と結晶化前処理

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

DOI:
10.3791/200736-v

•

02:35 min

•

August 25, 2023

•

, , ,
1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, 2Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

Tags

Keywords: Reductive MethylationABCG5/G8 ProteinPurificationPre-crystallization TreatmentNickel-NTA ColumnPD-10 DesaltingCholesterol AdditionUltracentrifugationProtein ConcentrationAnalytical Gel Filtration

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/200736?article_type=v

脂質バイセル環境下でのABCG5/G8結晶化によるX線結晶構造解析
check_url/kr/200736?article_type=v

精製ヘテロ二量体ABCG5/G8タンパク質の還元的メチル化と結晶化前処理
check_url/kr/200736?article_type=v

吊り下げ式蒸気拡散法による脂質二量体ビセル中のヘテロ二量体ABCG5/G8タンパク質の結晶化

Read Article