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Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

English

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2,726 Views

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09:23 min

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March 10, 2023

DOI:

10.3791/64365-v

DOI:
10.3791/64365-v

•

09:23 min
March 10, 2023

•

31 Views
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1Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology,University Medical Center Utrecht, 2Amsterdam Cardiovascular Sciences, Department of Physiology,Amsterdam University Medical Center

Summary

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Presented here is a set of protocols for the generation and cryopreservation of cardiac spheroids (CSs) from human-induced pluripotent stem cell-derived cardiomyocytes cultured in a high-throughput, multidimensional format. This three-dimensional model functions as a robust platform for disease modeling, high-throughput screenings, and maintains its functionality after cryopreservation.

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Cardiac SpheroidsHuman-induced Pluripotent Stem CellsCardiomyocytesHigh-throughput ScreeningBiobankingCell CultureCell DissociationCell CountingSpheroid FormationSpheroid MaintenanceCryopreservation

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Maas, R. G. C., Beekink, T., Chirico, N., Snijders Blok, C. J. B., Dokter, I., Sampaio-Pinto, V., van Mil, A., Doevendans, P. A., Buikema, J. W., Sluijter, J. P. G., Stillitano, F. Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids. J. Vis. Exp. (193), e64365, doi:10.3791/64365 (2023).

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