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Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
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Biotechnik
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JoVE Journal Biotechnik
Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

2,453 Views

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09:23 min

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March 10, 2023

DOI:

10.3791/64365-v

DOI:
10.3791/64365-v

•

09:23 min
March 10, 2023

•

6 Views
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1Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology,University Medical Center Utrecht, 2Amsterdam Cardiovascular Sciences, Department of Physiology,Amsterdam University Medical Center

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Cardiac SpheroidsHuman-induced Pluripotent Stem CellsCardiomyocytesHigh-throughput ScreeningBiobankingCell CultureCell DissociationCell CountingSpheroid FormationSpheroid MaintenanceCryopreservation

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Maas, R. G. C., Beekink, T., Chirico, N., Snijders Blok, C. J. B., Dokter, I., Sampaio-Pinto, V., van Mil, A., Doevendans, P. A., Buikema, J. W., Sluijter, J. P. G., Stillitano, F. Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids. J. Vis. Exp. (193), e64365, doi:10.3791/64365 (2023).

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