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Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
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January 06, 2023
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The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.
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Rejnowicz, E., Wright, J., Veldman-Jones, M., Harborne, S. Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography. J. Vis. Exp. (191), e64322, doi:10.3791/64322 (2023).
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