Journal
/
Neuroscience
/
Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation

Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation

English

Automatically Generated

2,144 Views

•

07:16 min

•

December 09, 2022

DOI:

10.3791/64234-v

DOI:
10.3791/64234-v

•

07:16 min
December 09, 2022

•

30 Views
, , , ,
1Laboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center,Lund University, 2Lund Stem Cell Center,Lund University

Summary

Automatically generated

This protocol describes long-term organotypic cultures of adult human cortex combined with ex vivo intracortical transplantation of induced pluripotent stem cell-derived cortical progenitors, which present a novel methodology to further test stem cell-based therapies for human neurodegenerative disorders.

Explore More Videos

Organotypic CulturesAdult Human CortexEx Vivo ModelHuman Stem Cell TransplantationNeural CircuitryBrain DamageVibratomeCutting SolutionCoronal SlicesSagittal SlicesRinsing Solution

Read Article

Cite this Article

Palma-Tortosa, S., Martínez-Curiel, R., Aretio-Medina, C., Avaliani, N., Kokaia, Z. Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation. J. Vis. Exp. (190), e64234, doi:10.3791/64234 (2022).

Download .ris file

Copy

Share Video

.

Copy