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Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
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Neuroscience
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JoVE Journal Neuroscience
Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

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2,585 Views

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09:44 min

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May 20, 2022

DOI:

10.3791/63998-v

DOI:
10.3791/63998-v

•

09:44 min
May 20, 2022

•

11 Views
, , ,
1Graduate School of Biomedical Science and Engineering,University of Maine, 2Department of Neurosurgery,Ohio State University Wexner Medical Center, 3Boston Children’s Hospital,Harvard Medical School

Summary

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The ability to permanently mark stem cells and their progeny with a fluorophore using an inducible transgenic lineage tracing mouse line allows for spatial and temporal analysis of activation, proliferation, migration, and/or differentiation in vivo. Lineage tracing can reveal novel information about lineage commitment, response to intervention(s), and multipotency.

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Cite this Article

Jensen, G. S., Willows, J. W., Breault, D. T., Townsend, K. L. Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain. J. Vis. Exp. (183), e63998, doi:10.3791/63998 (2022).

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