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Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

English

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2,969 Views

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06:37 min

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June 15, 2022

DOI:

10.3791/63931-v

DOI:
10.3791/63931-v

•

06:37 min
June 15, 2022

•

18 Views
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1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science,Utrecht University, 2Department of Physics and Biophysics,University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Summary

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Differential dynamic microscopy (DDM) combines features of dynamic light scattering and microscopy. Here, the process of using DDM to characterize reconstituted cytoskeleton networks by quantifying the subdiffusive and caged dynamics of particles in vimentin networks and the ballistic motion of active myosin-driven actin-microtubule composites is presented.

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Cite this Article

Verwei, H. N., Lee, G., Leech, G., Petitjean, I. I., Koenderink, G. H., Robertson-Anderson, R. M., McGorty, R. J. Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy. J. Vis. Exp. (184), e63931, doi:10.3791/63931 (2022).

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