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Quantificazione della dinamica del citoscheletro mediante microscopia dinamica differenziale
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
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Quantificazione della dinamica del citoscheletro mediante microscopia dinamica differenziale

italiano

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2,999 Views

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06:37 min

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June 15, 2022

DOI:

10.3791/63931-v

DOI:
10.3791/63931-v

•

06:37 min
June 15, 2022

•

18 Views
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1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science,Utrecht University, 2Department of Physics and Biophysics,University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Summary

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La microscopia dinamica differenziale (DDM) combina caratteristiche di diffusione dinamica della luce e microscopia. Qui, viene presentato il processo di utilizzo del DDM per caratterizzare le reti citoscheletriche ricostituite quantificando la dinamica subdiffusiva e ingabbiata delle particelle nelle reti di vimentine e il movimento balistico dei compositi actina-microtubulo attivi guidati dalla miosina.

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Cite this Article

Verwei, H. N., Lee, G., Leech, G., Petitjean, I. I., Koenderink, G. H., Robertson-Anderson, R. M., McGorty, R. J. Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy. J. Vis. Exp. (184), e63931, doi:10.3791/63931 (2022).

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