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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

English

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3,866 Views

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11:02 min

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February 12, 2022

DOI:

10.3791/62844-v

DOI:
10.3791/62844-v

•

11:02 min
February 12, 2022

•

11 Views
,
1Department of Biology,Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital,Warren Alpert Medical School of Brown University

Summary

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We describe a dissection technique that preserves the architecture of the neuromuscular junction and enables a detailed immunocytochemical study of motor neurons in the adult Drosophila leg.

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Stilwell, G., Agudelo, A. Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron. J. Vis. Exp. (180), e62844, doi:10.3791/62844 (2022).

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