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A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

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4,719 Views

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07:08 min

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April 06, 2021

DOI:

10.3791/62487-v

DOI:
10.3791/62487-v

•

07:08 min
April 06, 2021

•

6 Views
, , ,
1Luminex Corporation, 2Departments of Pathology and Laboratory Medicine, Clinical Microbiology,University of Rochester Medical Center, 3Department of Microbiology and Immunology,University of Rochester Medical Center

Summary

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A flow analysis system for bead-based multiplexed assays which provides a two-reporter readout was used for the development of multiplex serological and antibody neutralization assays that can simultaneously measure neutralizing IgG and IgM antibodies for SARS-CoV-2.

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SARS-CoV-2Neutralization AssayIgGIgMMultiplexBead-basedFlow AnalysisDual ReporterAntigen-specificAntibody IsotypingPost-translational ModificationsFree Versus Bound Drug FormsLuminex

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Angeloni, S., Cameron, A., Pecora, N. D., Dunbar, S. A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System. J. Vis. Exp. (170), e62487, doi:10.3791/62487 (2021).

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