ジャーナル
/
免疫学と感染
/
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

4,705 Views

•

07:08 min

•

April 06, 2021

DOI:

10.3791/62487-v

DOI:
10.3791/62487-v

•

07:08 min
April 06, 2021

•

6 Views
, , ,
1Luminex Corporation, 2Departments of Pathology and Laboratory Medicine, Clinical Microbiology,University of Rochester Medical Center, 3Department of Microbiology and Immunology,University of Rochester Medical Center

概要

Automatically generated

A flow analysis system for bead-based multiplexed assays which provides a two-reporter readout was used for the development of multiplex serological and antibody neutralization assays that can simultaneously measure neutralizing IgG and IgM antibodies for SARS-CoV-2.

Explore More Videos

SARS-CoV-2Neutralization AssayIgGIgMMultiplexBead-basedFlow AnalysisDual ReporterAntigen-specificAntibody IsotypingPost-translational ModificationsFree Versus Bound Drug FormsLuminex

Read Article

Cite this Article

Angeloni, S., Cameron, A., Pecora, N. D., Dunbar, S. A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System. J. Vis. Exp. (170), e62487, doi:10.3791/62487 (2021).

Download .ris file

Copy

Share Video

.

Copy