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Immunology and Infection
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Investigating the Phagocytosis of Leishmania using Confocal Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

English

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2,472 Views

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08:41 min

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July 29, 2021

DOI:

10.3791/62459-v

DOI:
10.3791/62459-v

•

08:41 min
July 29, 2021

•

6 Views
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1Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, 2National Institute of Science and Technology of Tropical Diseases – National Council for Scientific Research and Development (CNPq)

Summary

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The mechanism associated with phagocytosis in Leishmania infection remains poorly understood. Here, we describe methods to evaluate the early events occurring during Leishmania interaction with the host cells.

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PhagocytosisLeishmaniaConfocal MicroscopyTHP-1 CellsMacrophagesPromastigotesPV BiogenesisTransfection

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Cite this Article

Paixão, A. R., Dias, B. R. S., Palma, L. C., Tavares, N. M., Brodskyn, C. I., de Menezes, J. P. B., Veras, P. S. T. Investigating the Phagocytosis of Leishmania using Confocal Microscopy. J. Vis. Exp. (173), e62459, doi:10.3791/62459 (2021).

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