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Immunologie und Infektion
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Investigating the Phagocytosis of Leishmania using Confocal Microscopy
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

2,470 Views

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08:41 min

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July 29, 2021

DOI:

10.3791/62459-v

DOI:
10.3791/62459-v

•

08:41 min
July 29, 2021

•

6 Views
, , , , , ,
1Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, 2National Institute of Science and Technology of Tropical Diseases – National Council for Scientific Research and Development (CNPq)

Summary

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The mechanism associated with phagocytosis in Leishmania infection remains poorly understood. Here, we describe methods to evaluate the early events occurring during Leishmania interaction with the host cells.

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PhagocytosisLeishmaniaConfocal MicroscopyTHP-1 CellsMacrophagesPromastigotesPV BiogenesisTransfection

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Paixão, A. R., Dias, B. R. S., Palma, L. C., Tavares, N. M., Brodskyn, C. I., de Menezes, J. P. B., Veras, P. S. T. Investigating the Phagocytosis of Leishmania using Confocal Microscopy. J. Vis. Exp. (173), e62459, doi:10.3791/62459 (2021).

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