/
/
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
A subscription to JoVE is required to view this content. Sign in or start your free trial.
6,978 Views
•
07:42 min
•
April 16, 2021
DOI:
Summary
Automatically generated
A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.
Read Article
Cite this Article
Guallar-Garrido, S., Luquin, M., Julián, E. Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography. J. Vis. Exp. (170), e62368, doi:10.3791/62368 (2021).
Copy