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Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
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Biology
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JoVE Journal Biology
Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

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3,420 Views

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10:49 min

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April 13, 2021

DOI:

10.3791/62293-v

DOI:
10.3791/62293-v

•

10:49 min
April 13, 2021

•

11 Views
, , , , ,
1Institute of Physiology, Faculty of Medicine,University of Maribor, 2Faculty of Natural Sciences and Mathematics,University of Maribor, 3Center for Physiology and Pharmacology,Medical University of Vienna

Summary

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We present the preparation of acute pancreatic tissue slices and their use in confocal laser scanning microscopy to study calcium dynamics simultaneously in a large number of live cells, over long time periods, and with high spatiotemporal resolution.

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Cite this Article

Stožer, A., Dolenšek, J., Križančić Bombek, L., Pohorec, V., Slak Rupnik, M., Klemen, M. S. Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices. J. Vis. Exp. (170), e62293, doi:10.3791/62293 (2021).

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