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A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging
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A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

English

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3,182 Views

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06:29 min

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February 15, 2021

DOI:

10.3791/62227-v

DOI:
10.3791/62227-v

•

06:29 min
February 15, 2021

•

6 Views
, ,
1Department of Plant and Soil Sciences,University of Delaware, 2National Synchrotron Radiation Lightsource-II,Brookhaven National Laboratory

Summary

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We describe a protocol to sample, preserve, and section intact roots and the surrounding rhizosphere soil from wetland environments using rice (Oryza sativa L.) as a model species. Once preserved, the sample can be analyzed using elemental imaging techniques, such as synchrotron X-ray fluorescence (XRF) chemical speciation imaging.

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Cite this Article

Seyfferth, A. L., Limmer, M. A., Tappero, R. A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging. J. Vis. Exp. (168), e62227, doi:10.3791/62227 (2021).

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