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In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
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Neuroscience
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JoVE Journal Neuroscience
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

English

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4,319 Views

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05:25 min

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February 10, 2021

DOI:

10.3791/62166-v

DOI:
10.3791/62166-v

•

05:25 min
February 10, 2021

•

7 Views
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1Cellular and Molecular Neurobiology, Zoological Institute,Technische Universität Braunschweig, 2Institute of Pathophysiology,University Medical Center of the Johannes Gutenberg University, 3Cell Physiology, Zoological institute,Technische Universität Braunschweig

Summary

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Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention.

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Cite this Article

Schramm, P., Hetsch, F., Meier, J. C., Köster, R. W. In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal. J. Vis. Exp. (168), e62166, doi:10.3791/62166 (2021).

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