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Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

English

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4,868 Views

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14:48 min

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March 21, 2021

DOI:

10.3791/62097-v

DOI:
10.3791/62097-v

•

14:48 min
March 21, 2021

•

3 Views
, ,
1Small Animal Clinical Sciences, Western College of Veterinary Medicine,University of Saskatchewan

Summary

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Combined ozone and bacterial endotoxin exposed mice show wide-spread cell death, including that of neutrophils. We observed cellular adaptations such as disruption of cytoskeletal lamellipodia, increased cellular expression of complex V ATP synthase subunit β and angiostatin in broncho-alveolar lavage, suppression of the lung immune response and delayed neutrophil recruitment.

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Duda, J. A. B., Kaur, M., Aulakh, G. K. Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury. J. Vis. Exp. (169), e62097, doi:10.3791/62097 (2021).

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