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Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
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Neuroscience
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JoVE Journal Neuroscience
Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

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3,321 Views

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04:47 min

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May 22, 2020

DOI:

10.3791/61237-v

DOI:
10.3791/61237-v

•

04:47 min
May 22, 2020

•

1 Views
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1Department of Pharmacology,University of Colorado Denver School of Medicine

Summary

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Here, we present an optimized protocol to rapidly and semiquantitatively measure ligand-receptor interactions in trans in a heterologous cell system using fluorescence microscopy.

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Protein AggregationTranscellular InteractionsCell-based AssayTrans AdhesionHeterologous Cell SystemHEK-293T CellsEDTAHemocytometerFluorescence ImagingProtein Of InterestLigand Of InterestSynaptic Ligand

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Restrepo, S., Schwartz, S. L., Kennedy, M. J., Aoto, J. Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System. J. Vis. Exp. (159), e61237, doi:10.3791/61237 (2020).

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