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Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
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Biology
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Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart

Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart

English

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6,875 Views

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11:53 min

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June 14, 2020

DOI:

10.3791/61224-v

DOI:
10.3791/61224-v

•

11:53 min
June 14, 2020

•

47 Views
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1San Diego State University Heart Institute and the Department of Biology,San Diego State University

Summary

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A method is described for the simultaneous isolation of myocytes and non-myocytes from both the atria and ventricles of a single adult mouse heart. This protocol results in consistent yields of highly viable cardiac myocytes and non-myocytes and details optimal cell-specific culture conditions for phenotyping and in vitro analysis.

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Cite this Article

Blackwood, E. A., Bilal, A. S., Azizi, K., Sarakki, A., Glembotski, C. C. Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart. J. Vis. Exp. (160), e61224, doi:10.3791/61224 (2020).

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