Journal
/
Neuroscience
/
Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons

Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons

English

Automatically Generated

10,066 Views

•

10:03 min

•

August 16, 2020

DOI:

10.3791/61118-v

DOI:
10.3791/61118-v

•

10:03 min
August 16, 2020

•

21 Views
, , , ,
1Institute of Biotechnology, HiLIFE,University of Helsinki, 2Neuroscience Center, HiLIFE,University of Helsinki, 3Department of Brain Biochemistry, Maj Institute of Pharmacology,Polish Academy of Sciences

Summary

Automatically generated

Here, we present a detailed protocol to study neuronal α-synuclein accumulation in primary mouse dopamine neurons. Phosphorylated α-synuclein aggregates in neurons are induced with pre-formed α-synuclein fibrils. Automated imaging of immunofluorescently labeled cells and unbiased image analysis make this robust protocol suitable for medium-to-high throughput screening of drugs that inhibit α-synuclein accumulation.

Explore More Videos

Alpha-synucleinPreformed FibrilsPrimary Dopamine NeuronsParkinson’s DiseaseNeurodegenerative DiseaseProtein AccumulationCell CultureHigh-throughput ScreeningImage AnalysisNeuron FunctionMolecular Mechanisms

Read Article

Cite this Article

Er, S., Hlushchuk, I., Airavaara, M., Chmielarz, P., Domanskyi, A. Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons. J. Vis. Exp. (162), e61118, doi:10.3791/61118 (2020).

Download .ris file

Copy

Share Video

.

Copy