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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

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5,268 Views

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08:02 min

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January 22, 2020

DOI:

10.3791/60585-v

DOI:
10.3791/60585-v

•

08:02 min
January 22, 2020

•

2 Views
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1Division of Regenerative Medicine, Department of Medicine,Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine,Long Island University, 4Jinan Infectious Disease Hospital,Shandong University

Summary

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Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.

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Bi, H., Wasnik, S., Baylink, D. J., Liu, C., Tang, X. In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction. J. Vis. Exp. (155), e60585, doi:10.3791/60585 (2020).

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