/
/
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
A subscription to JoVE is required to view this content. Sign in or start your free trial.
7,290 Views
•
13:02 min
•
December 28, 2019
DOI:
Summary
Automatically generated
We describe protocols for the structure determination of the IKK-binding domain of NEMO by X-ray crystallography. The methods include protein expression, purification and characterization as well as strategies for successful crystal optimization and structure determination of the protein in its unbound form.
Read Article
Cite this Article
Barczewski, A. H., Ragusa, M. J., Mierke, D. F., Pellegrini, M. Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO. J. Vis. Exp. (154), e60339, doi:10.3791/60339 (2019).
Copy