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Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
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生化学
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JoVE Journal 生化学
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

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13:02 min

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December 28, 2019

DOI:

10.3791/60339-v

DOI:
10.3791/60339-v

•

13:02 min
December 28, 2019

•

3 Views
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1化学科,Dartmouth College

概要

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We describe protocols for the structure determination of the IKK-binding domain of NEMO by X-ray crystallography. The methods include protein expression, purification and characterization as well as strategies for successful crystal optimization and structure determination of the protein in its unbound form.

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Cite this Article

Barczewski, A. H., Ragusa, M. J., Mierke, D. F., Pellegrini, M. Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO. J. Vis. Exp. (154), e60339, doi:10.3791/60339 (2019).

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