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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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Genetics
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JoVE Journal Genetics
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

English

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7,117 Views

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00:09 min

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August 21, 2019

DOI:

10.3791/59901-v

DOI:
10.3791/59901-v

•

00:09 min
August 21, 2019

•

1 Views
, ,
1Division of Medical Genetics,University of Washington School of Medicine

Summary

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Here, we describe a step-by-step strategy for isolating small RNAs, enriching for microRNAs, and preparing samples for high-throughput sequencing. We then describe how to process sequence reads and align them to microRNAs, using open source tools.

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Cite this Article

Course, M. M., Gudsnuk, K., Valdmanis, P. N. A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools. J. Vis. Exp. (150), e59901, doi:10.3791/59901 (2019).

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