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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
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JoVE Journal Genetics
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

English

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9,139 Views

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10:00 min

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July 11, 2019

DOI:

10.3791/59720-v

DOI:
10.3791/59720-v

•

10:00 min
July 11, 2019

•

8 Views
, , , , , , , , ,
1Department of Biological Sciences,University of Alabama, 2Department of Genetics,University of Wisconsin-Madison

Summary

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We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.

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Cite this Article

Gevedon, O., Bolus, H., Lye, S. H., Schmitz, K., Fuentes-González, J., Hatchell, K., Bley, L., Pienaar, J., Loewen, C., Chtarbanova, S. In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster. J. Vis. Exp. (149), e59720, doi:10.3791/59720 (2019).

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