Journal
/
Neuroscience
/
A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

English

Automatically Generated

12,334 Views

•

12:49 min

•

September 28, 2019

DOI:

10.3791/59444-v

DOI:
10.3791/59444-v

•

12:49 min
September 28, 2019

•

7 Views
, , , , , ,
1Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology, 2Visual Computing Center,King Abdullah University of Science and Technology

Summary

Automatically generated

The described pipeline is designed for the segmentation of electron microscopy datasets larger than gigabytes, to extract whole-cell morphologies. Once the cells are reconstructed in 3D, customized software designed around individual needs can be used to perform a qualitative and quantitative analysis directly in 3D, also using virtual reality to overcome view occlusion.

Explore More Videos

3D ReconstructionVirtual Reality AnalysisGlial CellsNeuronal CellsAutomated Serial Section Electron MicroscopyImage ProcessingSegmentationTrakEM2Ilastik3D ModelingStructural ImpairmentsDiagnostic StrategyImage StitchingImage Downsampling

Read Article

Cite this Article

Calì, C., Kare, K., Agus, M., Veloz Castillo, M. F., Boges, D., Hadwiger, M., Magistretti, P. A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells. J. Vis. Exp. (151), e59444, doi:10.3791/59444 (2019).

Download .ris file

Copy

Share Video

.

Copy