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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genetics
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JoVE Journal Genetics
Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

English

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20,356 Views

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07:56 min

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April 11, 2019

DOI:

10.3791/59086-v

DOI:
10.3791/59086-v

•

07:56 min
April 11, 2019

•

16 Views
, , ,
1Genome Institute of Singapore,Agency for Science Technology and Research, 2School of Chemical and Biomedical Engineering,Nanyang Technological University

Summary

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CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.

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Cite this Article

Liu, K. I., Sutrisnoh, N. B., Wang, Y., Tan, M. H. Genome Editing in Mammalian Cell Lines using CRISPR-Cas. J. Vis. Exp. (146), e59086, doi:10.3791/59086 (2019).

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