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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Kapitel
- 00:04Titel
- 00:18Cell Passaging
- 01:08Cell Transfection
- 01:47Transfected Cell Fluorescence Activated Cell Sorting (FACS)
- 02:32Individual Clone Expansion and Editing Efficiency Evaluation
- 06:09Results: Representative Gene Editing Efficiency Analyses
- 07:24Conclusion
CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.
