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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
JoVE Journal
Genetics
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JoVE Journal Genetics
Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

English

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13,338 Views

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05:52 min

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January 07, 2019

DOI:

10.3791/58679-v

DOI:
10.3791/58679-v

•

05:52 min
January 07, 2019

•

11 Views
, , , ,
1Wellcome Sanger Institute, Wellcome Genome Campus, 2Department of Medicine,University of Cambridge

Summary

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We demonstrate the use of discontinuous density gradients to separate bacterial populations based on capsule production. This method is used to compare capsule amount between cultures, isolate mutants with a specific capsule phenotype, or to identify capsule regulators. Described here is the optimization and running of the assay.

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Cite this Article

Feltwell, T., Dorman, M. J., Goulding, D. A., Parkhill, J., Short, F. L. Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient. J. Vis. Exp. (143), e58679, doi:10.3791/58679 (2019).

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