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Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

English

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24,860 Views

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12:03 min

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April 18, 2019

DOI:

10.3791/58636-v

DOI:
10.3791/58636-v

•

12:03 min
April 18, 2019

•

24 Views
,
1Department of Pathology,Beth Israel Deaconess Medical Center, 2Division of Infectious Diseases,Boston Children’s Hospital, 3Harvard Medical School

Summary

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Antimicrobial synergy testing is used to evaluate the effect of two or more antibiotics used in combination and is typically performed by one of two methods: the checkerboard array or the time-kill assay. Here, we present an automated, inkjet printer-assisted checkerboard array synergy technique and a classic time-kill synergy study.

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Brennan-Krohn, T., Kirby, J. E. Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method. J. Vis. Exp. (146), e58636, doi:10.3791/58636 (2019).

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