Here, we present a protocol for generating neutrophil extracellular traps (NETs) and operating NETQUANT, a fully automatic software option for quantification of NETs in immunofluorescence images.
Mohanty, T., Nordenfelt, P. Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT. J. Vis. Exp. (153), e58528, doi:10.3791/58528 (2019).